Open AccessEffect of dietary protein reduction with lysine and methionnine supplementation on growth performance, body composition and total ammonia nitrogen excretion of juvenile grass carp, C tenopharyngodon idella
Author(s) -
Gan L.,
Liu Y.J.,
Tian L.X.,
Yang H.J.,
Yue Y.R.,
Chen Y.J.,
Liang J.J.,
Liang G.Y.
Publication year - 2012
Publication title -
aquaculture nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.941
H-Index - 79
eISSN - 1365-2095
pISSN - 1353-5773
DOI - 10.1111/j.1365-2095.2012.00937.x
Subject(s) - methionine , grass carp , lysine , biology , feed conversion ratio , zoology , composition (language) , excretion , weight gain , protein efficiency ratio , food science , amino acid , fish meal , biochemistry , body weight , fish <actinopterygii> , endocrinology , fishery , linguistics , philosophy
A 63‐day growth trial was undertaken to estimate the effects of supplemented lysine and methionine with different dietary protein levels on growth performance and feed utilization in Grass Carp ( C tenopharyngodon idella ). Six plant‐based practical diets were prepared, and 32 CP , 30 CP and 28 CP diets were formulated to contain 320 g kg −1 , 300 g kg −1 and 280 g kg −1 crude protein without lysine and methionine supplementation. In the supplementary group, lysine and methionine were added to formulate 32 AA , 30 AA and 28 AA diets with 320 g kg −1 , 300 g kg −1 and 280 g kg −1 dietary crude protein, respectively, according to the whole body amino acid composition of Grass Carp. In the groups without lysine and methionine supplementation, weight gain ( WG , %) and specific growth rate ( SGR , % day −1 ) of the fish fed 32 CP diet were significantly higher than that of fish fed 30 CP and 28 CP diets, but no significant differences were found between 30 CP ‐ and 28 CP ‐diet treatments. WG and SGR of the fish fed 32 AA and 30 AA diets were significantly higher than that of fish fed 28 AA diets, and the performance of grass carp was also significantly improved when fed diets with lysine and methionine supplementation ( P < 0.05), and the interaction between dietary protein level and amino acid supplementation was noted between WG and SGR ( P < 0.05). Feed intake (FI) was significantly increased with the increase in dietary protein level and the supplementation of lysine and methionine ( P < 0.05), but feed conversion ratio ( FCR ) showed a significant decreasing trend ( P < 0.05). Two days after total ammonia nitrogen ( TAN ) concentration test, the values of TAN discharged by the fish 8 h after feeding were 207.1, 187.5, 170.6, 157.3, 141.3 and 128.9 mg kg −1 body weight for fish fed 32 CP , 32 AA , 30 CP , 30 AA , 28 CP and 28 AA diets, respectively. TAN excretion by grass carp was reduced in plant‐based practical diets with the increase in dietary protein level and the supplementation of lysine and methionine ( P < 0.05). The results indicated that lysine and methionine supplementation to the plant protein sources‐based practical diets can improve growth performance and feed utilization of grass carp, and the dietary crude protein can be reduced from 320 g kg −1 to 300 g kg −1 through balancing amino acids profile. The positive effect was not observed at 280 g kg −1 crude protein level.