Open AccessEffect of Lactococcus lactis and Enterococcus faecium on growth performance, digestive enzymes and immune response of grouper Epinephelus coioides
Author(s) -
SUN Y.Z.,
YANG H.L.,
MA R.L.,
SONG K.,
LI J.S.
Publication year - 2012
Publication title -
aquaculture nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.941
H-Index - 79
eISSN - 1365-2095
pISSN - 1353-5773
DOI - 10.1111/j.1365-2095.2011.00894.x
Subject(s) - biology , lysozyme , epinephelus , lactococcus lactis , enterococcus faecium , probiotic , grouper , streptococcus iniae , protease , immune system , microbiology and biotechnology , food science , lactic acid , zoology , bacteria , enzyme , biochemistry , immunology , fishery , genetics , fish <actinopterygii> , antibiotics
The effect of dietary administration of Lactococcus lactis MM1 and Enterococcus faecium MM4, two strains of lactic acid bacteria isolated from the gut of juvenile grouper Epinephelus coioides , on the growth, feed utilization, digestive enzymes and immune response of juvenile E. coioides was evaluated. The fish were fed for 60 days with three different diets: control diet (without probiotic), diet P1 supplemented with 1.0 × 10 8 CFU g −1 L. lactis or diet P2 with 1.0 × 10 8 CFU g −1 E. faecium . Although growth and feed intake decreased slightly, significant improvement of feed utilization was observed in fish fed diet P1 and diet P2 for 60 days. The hepatopancreatic protease activities in the two probiotic treatments were significantly higher than that in the control. The phagocytic activity, serum superoxide dismutase activity and complement component 4 (C4) and IgM levels were not significantly affected by diet P1 and diet P2. Serum complement component 3 (C3) levels in the two treatments were significantly higher than that in the control. Significant increased serum lysozyme activity was observed in fish fed the diet P1, while significant decreased lysozyme activity was observed in fish fed the diet P2 compared with the fish fed the control diet. There was a significant interaction effect between diet and administration date for the complement C4. The study demonstrates potential for L. lactis MM1 and E. faecium MM4 to improve the feed utilization and modulate the immune function in E. coioides .