Open AccessEffects of dietary pyridoxine on disease resistance, immune responses and intestinal microflora in juvenile Jian carp ( Cyprinus carpio var. Jian)
Author(s) -
FENG L.,
HE W.,
JIANG J.,
LIU Y.,
ZHOU X.Q.
Publication year - 2010
Publication title -
aquaculture nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.941
H-Index - 79
eISSN - 1365-2095
pISSN - 1353-5773
DOI - 10.1111/j.1365-2095.2009.00660.x
Subject(s) - biology , pyridoxine , aeromonas hydrophila , carp , cyprinus , immune system , lysozyme , medicine , microbiology and biotechnology , endocrinology , zoology , immunology , bacteria , biochemistry , fish <actinopterygii> , fishery , genetics
This experiment was conducted to evaluate the effects of dietary pyridoxine on disease resistance, immune responses and intestinal microflora of fish. A total of 1050 Jian carp (11.71 ± 0.05 g) were randomly distributed into seven groups, feeding diets containing graded levels of pyridoxine (0.2, 1.7, 3.2, 5.0, 6.3, 8.6 and 12.4 mg kg −1 diet). After 80 days of feeding, a challenge trial was conducted by injection of Aeromonas hydrophila for 17 days. Results indicated that with increasing dietary pyridoxine concentration up to 5.0 mg kg −1 diet, survival rate after challenge with A.hydrophila and phagocytic activity of leukocyte were improved ( P < 0.05), and plateaued thereafter ( P > 0.05). Red blood cell and white blood cell counts were lowest when fed the diet containing 1.7 mg pyridoxine kg −1 diet. Haemagglutination titre, lysozyme activity, acid phosphatase activity, total iron‐binding capacity, antibody titre and immunoglobulin M content followed the similar pattern to that observed with survival rate. Aeromonas hydrophila , Escherichia coli and Lactobacillus counts in intestine were not affected by dietary pyridoxine concentration ( P > 0.05). These results suggested that pyridoxine could enhance immune response of fish.