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Polymorphism identification in goat GNRH1 and GDF9 genes and their association analysis with litter size
Author(s) -
An X. P.,
Hou J. X.,
Zhao H. B.,
Li G.,
Bai L.,
Peng J. Y.,
M Yan Q.,
Song Y. X.,
Wang J. G.,
Cao B. Y.
Publication year - 2013
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.2012.02394.x
Subject(s) - biology , litter , genetics , genotype , allele , gene , missense mutation , snp , polymorphism (computer science) , microbiology and biotechnology , single nucleotide polymorphism , mutation , ecology
Summary This study investigated the polymorphisms of GNRH1 and GDF9 genes in 641 goats of three breeds: X inong S aanen, G uanzhong and B oer. Two allelic variants were identified in the GNRH1 gene ( JN645280 :g.3548A>G and JN645281 :g.3699G>A) and one allelic variant was found in the GDF9 gene ( JN655693 :g.4093G>A). Furthermore, g.4093G>A was a missense mutation (p.Val397Ile of GDF9 ). Results of an association analysis indicated that SNP s g.3548A>G and g.4093G>A had significant effects on litter size ( P < 0.05). The combination genotypes of SNP s g.3548A>G, g.3699G>A and g.4093G>A also affected litter size with the C 5 genotype having the highest litter size in the first, third, fourth and average parity. Hence, the biochemical and physiological functions, together with the results obtained in our investigation, suggest that the GNRH1 and GDF9 genes could serve as genetic markers for litter size in goat breeding.