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Identification and comparison of micro RNA s from skeletal muscle and adipose tissues from two porcine breeds
Author(s) -
Li HongYi,
Xi QianYun,
Xiong YuanYan,
Liu XiaoLong,
Cheng Xiao,
Shu Gang,
Wang SongBo,
Wang LiNa,
Gao Ping,
Zhu XiaoTong,
Jiang QingYan,
Yuan Li,
Zhang YongLiang
Publication year - 2012
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.2012.02332.x
Subject(s) - rna , biology , adipose tissue , skeletal muscle , gene expression , messenger rna , microrna , gene , microbiology and biotechnology , rna extraction , small rna , genetics , biochemistry , endocrinology
Summary Micro RNA s (mi RNA s) are an abundant class of small regulatory RNA s that negatively regulate gene expression at the post‐transcriptional level. Although an increasing number of porcine mi RNA s recently have been identified, research has yet to identify the full repertoire of mi RNA s in pig skeletal and adipose tissues and their differences between breeds. We extracted small RNA from skeletal muscle and adipose tissues of L andrace and L antang pigs, and the expression of a total of 184 known porcine mi RNA s (113 from Solexa sequencing and 171 from mi RNA chip hybridization) as well as 521 novel mi RNA candidates was detected. Moreover, 20 mi RNA s were selected randomly from the 184 mi RNA s and analysed by quantitative real‐time PCR to confirm the aforementioned results. In the skeletal muscle tissues, 21 mi RNA s were up‐regulated in Lantang and another 33 were highly expressed in Landrace pigs. In the adipose tissues, 25 mi RNA s were down‐regulated in L antang and another 23 were lowly expressed in L andrace pigs. mi RNA divergence between tissues was also detected in this study. Ten mi RNA s were highly expressed in the skeletal muscle tissue in comparison with adipose tissue, and another 10 mi RNA s exhibited the opposite expression profile. To investigate the regulatory mechanism of the mi RNA s in muscle and adipose tissues, the 10 mi RNA s with the most divergent expression profiles were functionally categorized using the K yoto E ncyclopedia of G enes and G enomes database. Most of the mi RNA s strongly corresponded to myogenesis and adipogenesis processes. In addition, 84 of the 521 mi RNA candidates were potentially porcine‐specific mi RNA s. This study adds new valuable information to comparative mi RNA profiles of skeletal muscle and adipose tissues in porcine species. The great diversity of mi RNA composition and expression levels both between breeds and between tissues suggests that a complex regulatory network exists in porcine subcutaneous fat development.