Premium
A novel USP9Y polymorphism allowing a rapid and unambiguous classification of Bos taurus Y chromosomes into haplogroups
Author(s) -
Bonfiglio S.,
De Gaetano A.,
Tesfaye K.,
Grugni V.,
Semino O.,
Ferretti L.
Publication year - 2012
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.2012.02328.x
Subject(s) - haplogroup , biology , genetics , genbank , typing , sequence (biology) , allele , gene , haplotype
Summary A new sequence‐tagged site ( STS ) was identified within intron 26 of the bovine USP9Y gene, showing an 81‐base pair insertion (g.76439_76440ins81 in sequence with GenBank accession FJ195366 ) able to distinguish Y 2 and Y 3 Bos Y haplogroups from Y 1. Moreover, four Y 3‐specific sequence variants allow a distinction from haplogroup Y 2. The typing of a Bison bison Y chromosome indicates that the ancestral allele for the USP9Y 81‐bp insertion is the short Y 1 version. The results from typing the new STS in 1230 cattle Y chromosomes are fully consistent with their classification through standard methods. Thanks to the newly identified STS , it is now possible to assign cattle Y chromosomes to the currently known haplogroups using a single marker.