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Association of the polymorphism g.8514C>T in the osteopontin gene ( SPP1 ) with milk yield in the dairy cattle breed Girolando
Author(s) -
Mello F.,
Cobuci J. A.,
Martins M. F.,
Silva M. V. G. B.,
Neto J. B.
Publication year - 2012
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.2011.02312.x
Subject(s) - breed , biology , osteopontin , dairy cattle , genetics , immunology
Source/description: The genomic DNA of 434 Girolando animals was amplified for the fourth intron of the osteopontin gene (SPP1) (HUGO nomenclature: secreted phosphoprotein 1) and then digested with restriction enzyme BsrI to detect the alleles of this SNP by PCR-RFLP. Three genotypes were identified in the population, TT, CT and CC (52.53%, 38.71% and 8.76%), respectively, and these were used for an association study with data on 305 day milk yield (M305) and predicted transmitting ability for milk yield (PTAM). Recorded milk data were from the Dairy Control Service of the Brazilian Association of Breeders Girolando managed by Embrapa Dairy Cattle. The predicted values of transmitting ability for milk (PTAM) for bulls and their daughters were taken from the national breed genetic evaluation programme in 2009 by Embrapa; these values are adjusted in relation to the genetic basis (7.59 kg), defined as the mean genetic 646 cows born in 2000.