Premium
A genome‐wide survey of copy number variation regions in various chicken breeds by array comparative genomic hybridization method
Author(s) -
Wang Y.,
Gu X.,
Feng C.,
Song C.,
Hu X.,
Li N.
Publication year - 2012
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.2011.02308.x
Subject(s) - biology , copy number variation , comparative genomic hybridization , genetics , locus (genetics) , quantitative trait locus , copy number analysis , genome , gene , genomic dna , computational biology
Summary The discovery of copy number variation ( CNV ) in the genome has provided new insight into genomic polymorphism. Studies with chickens have identified a number of large CNV segments using a 385k comparative genomic hybridization ( CGH ) chip (mean length >140 kb). We present a detailed CNV map for local Chinese chicken breeds and commercial chicken lines using an Agilent 400k array CGH platform with custom‐designed probes. We identified a total of 130 copy number variation regions ( CNVR s; mean length = 25.70 kb). Of these, 104 (80.0%) were novel segments reported for the first time in chickens. Among the 104 novel CNVR s, 56 (53.8%) of the segments were non‐coding sequences, 65 (62.5%) showed the gain of DNA and 40 (38.5%) showed the loss of DNA (one locus showed both loss and gain). Overlapping with the formal selective sweep data and the quantitative trait loci data, we identified four loci that might be considered to be high‐confidence selective segments that arose during the domestication of chickens. Compared with the CNVR s reported previously, genes for the positive regulation of phospholipase A 2 activity were discovered to be significantly over‐represented in the novel CNVR s reported here by gene ontology analysis. Availability of our results should facilitate further research in the study of the genetic variability in chicken breeds.