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Identification of BoLA‐DRB3.2 alleles in Korean native cattle (Hanwoo) and Holstein populations using a next generation sequencer
Author(s) -
Lee BoYoung,
Hur TaiYoung,
Jung YoungHun,
Kim Heebal
Publication year - 2012
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.2011.02264.x
Subject(s) - biology , allele , hanwoo , genetics , genotyping , genotype , typing , computational biology , gene , food science
Summary Bovine leucocyte antigen (encoded by BoLA ) has been widely studied to identify the association with many traits related to immunity. Exon2 of BoLA‐DRB 3 is extremely polymorphic, and more than 100 alleles have been identified. We investigated polymorphisms of BoLA‐DRB3.2 in Korean native cattle and Holstein populations using a next generation sequencer of the GS‐FLX Titanium system. We found 38 alleles including 11 new alleles ( BoLA‐DRB3*1303, *4702, *7101, *7501, *7201, *7301, *7601, *1104, *7701, *7401 and *50021 ) in Hanwoo, and nine alleles including one new allele ( BoLA‐DRB3*7601 ) in Holstein. The 454 sequencing method is a promising alternative technology for high throughput genotyping of BoLA‐DRB3.2 because of its technical advantages that allow it to overcome the disadvantages of sequence‐based typing methods.