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Four genes located on a SSC2 meat quality QTL region are associated with different meat quality traits in Landrace × Chinese‐European crossbred population
Author(s) -
Čepica S.,
Óvilo C.,
Masopust M.,
Knoll A.,
Fernandez A.,
Lopez A.,
Rohrer G. A.,
neman D.
Publication year - 2012
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.2011.02252.x
Subject(s) - intramuscular fat , quantitative trait locus , crossbreed , biology , genetics , population , allele , candidate gene , gene , zoology , medicine , environmental health
Summary Several quantitative trait loci (QTL) for different meat quality traits have been localized on the q arm of porcine chromosome 2 at position 55–78 cM. Association analyses were performed in a commercial Landrace × Chinese‐European (LCE) crossbred population ( n = 446) slaughtered at approximately 127 kg and an average age of 198 days with records for performance (growth, fat and meat accretion) and meat quality [intramuscular fat (IMF), Minolta L* , Minolta a* , Minolta b* and pH at 45 m]. Polymorphisms within positional candidate genes cloned from homologous regions on human chromosome 19, ubiquitin‐like 5 ( UBL5 – AM950288:g.566G>A), resistin ( RETN – AM157180:g.1473A>G causing substitution p.Ala36Thr), insulin receptor ( INSR – AM950289:g.589T>C) and complement factor D (adipsin) ( CFD – AM950287:g. 306C>T) were located at positions 62.1, 64.0, 68.0 and 70.7 cM respectively on the current USDA USMARC map of porcine chromosome 2 and had the following allele frequencies in the LCE: UBL5 566G – 0.57; RETN 1473G – 0.84; INSR 589C – 0.70; and CFD 306C – 0.73. The effects of alleles within the candidate genes on the recorded traits were estimated using an animal model. Significant effects ( P < 0.05) were found for pH 45 in m. semimembranosus (m. sm.) ( UBL5 ), IMF ( RETN ) and Minolta L* ( RETN , CFD ). Differences between phenotypic means of homozygotes at UBL5 , RETN and either RETN or CFD explained 0.34 SD for pH 45 in m. sm., 0.47 SD for IMF and 0.68 SD for Minolta L * respectively. Suggestive effects ( P < 0.10) on IMF ( UBL5 , CFD ), Minolta a* ( INSR , CFD ) and Minolta b* ( INSR ) were also observed. Our results support the localization of further QTL for meat quality traits in this region and suggest that there are several genes affecting different meat quality traits.