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Polymorphism and gene organization of water buffalo MHC‐DQB genes show homology to the BoLA DQB region
Author(s) -
Sena L.,
Schneider M. P. C.,
Brenig B. B.,
Honeycutt R. L.,
Honeycutt D. A.,
Womack J. E.,
Skow L. C.
Publication year - 2011
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.2010.02157.x
Subject(s) - biology , bubalus , allele , genetics , major histocompatibility complex , phylogenetic tree , gene , water buffalo , single nucleotide polymorphism , genotype , veterinary medicine , medicine , ecology
Summary In cattle ( Bos taurus ), there is evidence of more than 50 alleles of BoLA‐DQB ( bovine lymphocyte antigen DQB ) that are distributed across at least five DQB loci, making this region one of the most complex in the BoLA gene family. In this study, DQB alleles were analysed for the water buffalo ( Bubalus bubalis ), another economically important bovine species. Twelve alleles for Bubu‐DQB ( Bubalis bubalis DQB ) were determined by nucleotide sequence analysis. A phylogenetic analysis revealed numerous trans‐species polymorphisms, with alleles from water buffalo assigned to at least three different loci ( BoLA‐DQB1 , BoLA‐DQB3 and BoLA‐DQB4 ) that are also found in cattle. These presumptive loci were analysed for patterns of synonymous ( d S ) and non‐synonymous ( d N ) substitution. Like BoLA ‐ DQB1 , Bubu‐DQB1 was observed to be under strong positive selection for polymorphism. We conclude that water buffalo and cattle share the current arrangement of their DQB region because of their common ancestry.