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Identification of equine major histocompatibility complex haplotypes using polymorphic microsatellites
Author(s) -
Tseng C. T.,
Miller D.,
Cassano J.,
Bailey E.,
Antczak D. F.
Publication year - 2010
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.2010.02125.x
Subject(s) - microsatellite , biology , haplotype , major histocompatibility complex , genotyping , typing , genetics , serotype , population , allele , genotype , antigen , virology , gene , demography , sociology
Summary A system for identifying equine major histocompatibility complex (MHC) haplotypes was developed based on five polymorphic microsatellites located within the MHC region on ECA 20. Molecular signatures for 50 microsatellite haplotypes were recognized from typing 353 horses. Of these, 23 microsatellite haplotypes were associated with 12 established equine leucocyte antigen (ELA) haplotypes in Thoroughbreds and Standardbreds. Five ELA serotypes were associated with multiple microsatellite subhaplotypes, expanding the estimates of diversity in the equine MHC. The strong correlations between serological and microsatellite typing demonstrated a linkage to known MHC class I protein polymorphisms and validated this assay as a useful supplement to ELA serotyping, and in some applications, a feasible alternative method for MHC genotyping in horse families and in population studies.