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Porcine NAMPT gene: search for polymorphism, mapping and association studies
Author(s) -
Čepica S.,
Bartenschlager H.,
Óvilo C.,
Zrůstová J.,
Masopust M.,
Fernández A.,
López A.,
Knoll A.,
Rohrer G. A.,
Snelling W. M.,
Geldermann H.
Publication year - 2010
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.2010.02052.x
Subject(s) - biology , linkage disequilibrium , genetics , intramuscular fat , single nucleotide polymorphism , population , gene , genotype , zoology , demography , sociology
Summary NAMPT encodes an enzyme catalysing the rate‐limiting step in NAD biosynthesis. The extracellular form of the enzyme is known as adipokine visfatin. We detected SNP AM999341:g.669T>C (referred to as 669T>C ) in intron 9 and SNP FN392209:g.358A>G (referred to as 358A>G ) in the promoter of the gene. RH mapping linked the gene to microsatellite SW944 . Linkage analysis placed the gene on the current USDA – USMARC linkage map at position 92 cM on SSC9. Association analyses were performed in a wild boar × Meishan F 2 family (W × M), with 45 traits recorded (growth and fattening, fat deposition, muscling, meat quality, stress resistance and other traits), and in a commercial Landrace × Chinese‐European (LCE) synthetic population with records for 15 traits (growth, fat deposition, muscling, intramuscular fat, meat colour and backfat fatty acid content). In the W × M, SNP 669T>C was associated with muscling, fat deposition, growth and fattening, meat quality and other traits and in the LCE with muscling, meat quality and backfat fatty acid composition. In the W × M, SNP 358A>G was associated with muscling, fat deposition, growth and other traits. After correction for multiple testing, the NAMPT haplotypes were associated in the W × M with, in descending order, muscling ( q = 0.0056), growth ( q = 0.0056), fat deposition ( q = 0.0109), fat‐to‐meat ratio ( q = 0.0135), cooling losses ( q = 0.0568) and longissimus pH U ( q = 0.0695). The SNPs are hypothesized to be in linkage disequilibrium with a causative mutation affecting energy metabolism as a whole rather than fat metabolism alone.