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Construction of a genetic linkage map for silver carp ( Hypophthalmichthys molitrix )
Author(s) -
Zhang L.,
Yang G.,
Guo S.,
Wei Q.,
Zou G.
Publication year - 2010
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.2010.02031.x
Subject(s) - microsatellite , biology , hypophthalmichthys , silver carp , genetic linkage , amplified fragment length polymorphism , genetics , linkage (software) , genome , gene , genetic diversity , population , fishery , allele , fish <actinopterygii> , demography , sociology
Summary For silver carp ( Hypophthalmichthys molitrix ), a combined microsatellite (or simple sequence repeat) and amplified fragment length polymorphism (AFLP) sex average linkage map was constructed. A total of 483 markers (245 microsatellites and 238 AFLPs) were assigned to 33 linkage groups. The map spanned 1352.2 cM, covering 86.4% of the estimated genome size of silver carp. The maximum and average spaces between 420 loci were 21.5 cM and 3.2 cM, respectively. The length of linkage groups ranged from 3.6 cM to 98.5 cM with an average of 41.0 cM. The number of markers per group varied from 2 to 44 with an average of 14.6. The AFLP markers significantly improved the integrity of microsatellite‐based linkage groups and increased the genome coverage and marker evenness. A genome‐wide recombination suppression was observed in male. In an extreme case, six microsatellites co‐segregated in male, but spanned a 45.1 cM region in female.