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Identification of a 3.7‐Mb region for a marbling QTL on bovine chromosome 4 by identical‐by‐descent and association analysis
Author(s) -
Yokouchi K.,
Mizoguchi Y.,
Watanabe T.,
Iwamoto E.,
Sugimoto Y.,
Takasuga A.
Publication year - 2009
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.2009.01956.x
Subject(s) - marbled meat , quantitative trait locus , biology , microsatellite , haplotype , genetics , population , chromosome , allele , gene , medicine , environmental health
Summary QTL mapping for growth and carcass traits was performed using a paternal half‐sib family composed of 325 Japanese Black cattle offspring. Nine QTL were detected at the 1% chromosome‐wise significance level at a false discovery rate of less than 0.1. These included two QTL for marbling on BTA 4 and 18, two QTL for carcass weight on BTA 14 and 24, two QTL for longissimus muscle area on BTA 1 and 4, two QTL for subcutaneous fat thickness on BTA 1 and 15 and one QTL for rib thickness on BTA 6. Although the marbling QTL on BTA 4 has been replicated with significant linkages in two Japanese Black cattle sires, the three Q (more marbling) haplotypes, each inherited maternally, were apparently different. To compare the three Q haplotypes in more detail, high‐density microsatellite markers for the overlapping regions were developed within the 95% CIs (65 markers in 44–78 cM). A detailed haplotype comparison indicated that a small region (<3.7 Mb) around 46 cM was shared between the Qs of the two sires, whose dams were related. An association of this region with marbling was shown by a regression analysis using the local population, in which the two sires were produced and this was confirmed by an association study using a population collected throughout Japan. These results strongly suggest that the marbling QTL on BTA 4 is located in the 3.7‐Mb region at around 46 cM.