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Molecular analysis of carbohydrate N‐acetylgalactosamine 4‐O sulfotransferase 8 ( CHST8 ) as a candidate gene for bovine spongiform encephalopathy susceptibility
Author(s) -
Morina R.,
Knorr C.,
Haase B.,
Leeb T.,
Seuberlich T.,
Zurbriggen A.,
Brem G.,
Schütz E.,
Brenig B.
Publication year - 2010
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.2009.01951.x
Subject(s) - bovine spongiform encephalopathy , biology , genotype , haplotype , allele , pathogenesis , gene , genetics , prion protein , disease , immunology , medicine
Summary Endogenous prion proteins (PrP) play the central role in the pathogenesis of transmissible spongiform encephalopathies. The carbohydrate N ‐acetylgalactosamine 4‐O sulfotransferase 8 (CHST8) promotes the conversion of the cellular PrP C into the pathogenic PrP d . Six sequence variants within the CHST8 gene were identified by comparative sequencing and genotyped for a sample of 623 animals comprising bovine spongiform encephalopathy (BSE)‐affected and healthy control cows representing German Fleckvieh (German Simmental), German Holstein (Holstein‐Friesian) and Brown Swiss. Significant differences in the allele, genotype and haplotype frequencies between BSE‐affected and healthy cows indicate an association of sequence variant g.37254017G>T with the development of the disease in Brown Swiss cattle.