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Parentage testing in alpacas ( Vicugna pacos ) using semi‐automated fluorescent multiplex PCRs with 10 microsatellite markers
Author(s) -
Agapito J.,
Rodríguez J.,
HerreraVelit P.,
Timoteo O.,
Rojas P.,
Boettcher P. J.,
García F.,
Espinoza J. R.
Publication year - 2008
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.2007.01697.x
Subject(s) - microsatellite , biology , genetics , loss of heterozygosity , locus (genetics) , offspring , genotype , allele , multiplex , multiplex polymerase chain reaction , population , polymerase chain reaction , gene , demography , pregnancy , sociology
Summary The aim of this study was to assess and apply a microsatellite multiplex system for parentage determination in alpacas. An approach for parentage testing based on 10 microsatellites was evaluated in a population of 329 unrelated alpacas from different geographical zones in Perú. All microsatellite markers, which amplified in two multiplex reactions, were highly polymorphic with a mean of 14.5 alleles per locus (six to 28 alleles per locus) and an average expected heterozygosity ( H E ) of 0.8185 (range of 0.698–0.946). The total parentage exclusion probability was 0.999456 for excluding a candidate parent from parentage of an arbitrary offspring, given only the genotype of the offspring, and 0.1 for excluding a candidate parent from parentage of an arbitrary offspring, given the genotype of the offspring and the other parent. In a case test of parentage assignment, the microsatellite panel assigned 38 (from 45 cases) offspring parentage to 10 sires with LOD scores ranging from 2.19 × 10 +13 to 1.34 × 10 +15 and Δ values ranging from 2.80 × 10 +12 to 1.34 × 10 +15 with an estimated pedigree error rate of 15.5%. The performance of this multiplex panel of markers suggests that it will be useful in parentage testing of alpacas.