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Construction of bovine whole‐genome radiation hybrid and linkage maps using high‐throughput genotyping
Author(s) -
McKay S. D.,
Schnabel R. D.,
Murdoch B. M.,
Aerts J.,
Gill C. A.,
Gao C.,
Li C.,
Matukumalli L. K.,
Stothard P.,
Wang Z.,
Van Tassell C. P.,
Williams J. L.,
Taylor J. F.,
Moore S. S.
Publication year - 2007
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.2006.01564.x
Subject(s) - genotyping , genome , bovine genome , biology , genetics , whole genome sequencing , multiplex , radiation hybrid mapping , sequence assembly , sequence tagged site , computational biology , dna sequencing , reference genome , snp genotyping , single nucleotide polymorphism , gene , gene mapping , genotype , transcriptome , chromosome , gene expression
Summary High‐density whole‐genome maps are essential for ordering genes or markers and aid in the assembly of genome sequence. To increase the density of markers on the bovine radiation hybrid map, and hence contribute to the assembly of the bovine genome sequence, an Illumina ® BeadStation was used to simultaneously type large numbers of markers on the Roslin‐Cambridge 3000 rad bovine–hamster whole‐genome radiation hybrid panel (WGRH 3000 ). In five multiplex reactions, 6738 sequence tagged site (STS) markers were successfully typed on the WGRH 3000 panel DNA. These STSs harboured SNPs that were developed as a result of the bovine genome sequencing initiative. Typically, the most time consuming and expensive part of creating high‐density radiation hybrid (RH) maps is genotyping the markers on the RH panel with conventional approaches. Using the method described in this article, we have developed a high‐density whole‐genome RH map with 4690 loci and a linkage map with 2701 loci, with direct comparison to the bovine whole‐genome sequence assembly (Btau_2.0) in a fraction of the time it would have taken with conventional typing and genotyping methods.