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Cross‐species overgo hybridization and comparative physical mapping within avian genomes
Author(s) -
Romanov M. N.,
Dodgson J. B.
Publication year - 2006
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.2006.01463.x
Subject(s) - zebra finch , biology , genome , genetics , comparative genomics , genomics , gene , comparative genomic hybridization , whole genome sequencing , neuroscience
Summary The chicken genome sequence facilitates comparative genomics within other avian species. We performed cross‐species hybridizations using overgo probes designed from chicken genomic and zebra finch expressed sequence tags (ESTs) to turkey and zebra finch BAC libraries. As a result, 3772 turkey BACs were assigned to 336 markers or genes, and 1662 zebra finch BACs were assigned to 164 genes. As expected, cross‐hybridization was more successful with overgos within coding sequences than within untranslated region, intron or flanking sequences and between chicken and turkey, when compared with chicken‐zebra finch or zebra finch‐turkey cross‐hybridization. These data contribute to the comparative alignment of avian genome maps using a ‘one sequence, multiple genomes’ strategy.