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High‐resolution physical mapping and construction of a porcine contig spanning the intramuscular fat content QTL
Author(s) -
Sato S.,
Hasebe H.,
Sato S.,
Asahi Y.,
Hayashi T.,
Kobayashi E.,
Sugimoto Y.
Publication year - 2006
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.2005.01397.x
Subject(s) - contig , biology , quantitative trait locus , intramuscular fat , resolution (logic) , computational biology , genetics , gene , food science , computer science , genome , artificial intelligence
Summary We previously mapped a locus for porcine intramuscular fat content (IMF) by linkage analysis to a 17.1‐cM chromosome interval on Sus scrofa chromosome 7 (SSC7) flanked by microsatellite markers SW1083 and SW581 . In this study, we identified 34 microsatellite markers and 14 STSs from the 17.1‐cM IMF quantitative trait loci (QTL) region corresponding to HSA14q and aligned those loci using the INRA‐University of Minnesota porcine radiation hybrid (IMpRH) panel. We then constructed a 5.2‐Mb porcine bacterial artificial chromosome (BAC) contig of this region that was aligned using the RH panel. Finally, the IMF QTL was fine‐mapped to 12.6 cM between SJ169 and MM70 at the 0.1% chromosome‐wise significance level by genotyping the previously studied F 2 resource family with 17 additional microsatellites. We also demonstrated that the SJ169 – MM70 interval spans approximately 3.0 Mb and contains at least 12 genes: GALC , GPR65 , KCNK10 , SPATA7 , PTPN21 , FLJ11806 , EML5 , TTC8 , CHES1 , CAP2P1 , CHORDC2P and C14orf143 .