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Malic enzyme 1 genotype is associated with backfat thickness and meat quality traits in pigs
Author(s) -
Vidal O.,
Varona L.,
Oliver M. A.,
Noguera J. L.,
Sànchez A.,
Amills M.
Publication year - 2006
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.2005.01366.x
Subject(s) - malic enzyme , biology , single nucleotide polymorphism , quantitative trait locus , genetics , genotype , locus (genetics) , haplotype , population , gene , untranslated region , snp , enzyme , biochemistry , rna , demography , sociology , dehydrogenase
Summary Malic enzyme 1 (ME1) is a part of the tricarboxylate shuttle that provides NADPH and acetyl‐CoA required in fatty acid biosynthesis. The pig ME1 locus maps on the proximal end of chromosome 1, where a quantitative trait loci (QTL) affecting fat deposition has been previously described. We amplified fragments of 1457 and 1459 bp that corresponded to the complete coding region and the 3′‐untranslated region (UTR), respectively, of the pig ME1 gene. The sequences of these two fragments in pigs from three breeds (Landrace, Large White and Piétrain) contained five single nucleotide polymorphisms (SNP) in the 3′‐UTR: C1706T, G1762T, A1807C, C1857A and T1880A. Three haplotypes were found in two generations of a selected Landrace population: H1 (C 1706 G 1762 A 1807 C 1857 A 1880 ), H2 (C 1706 G 1762 A 1807 C 1857 T 1880 ) and H3 (T 1706 T 1762 C 1807 A 1857 T 1880 ). Using Bayesian association analyses, significant associations (highest posterior density at 95%) between ME1 genotype and backfat (BF) thickness at 171 days and muscular pH were found in a Landrace population.