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Cloning and mapping of the porcine cytochrome‐p450 2E1 gene and its association with skatole levels in the domestic pig
Author(s) -
Skinner T. M.,
Doran E.,
McGivan J. D.,
Haley C. S.,
Archibald A. L.
Publication year - 2005
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.2005.01342.x
Subject(s) - skatole , biology , boar taint , single nucleotide polymorphism , genetics , snp , gene , microbiology and biotechnology , genotype , biochemistry , indole test
Summary The porcine cytochrome‐p450 2E1 ( CYP2E1 ) gene was isolated by screening a pig BAC library and partially sequenced. This sequence information was used to identify six single nucleotide polymorphisms (SNPs) within the CYP2E1 gene and its promoter. In addition, a microsatellite marker tightly linked to the CYP2E1 gene was subcloned from the BAC. One of these markers was used to map the CYP2E1 gene distal of SWC27 on SSC14, well outside reported quantitative trait loci on SSC14 for skatole, indole and taste test measures of boar taint. However, in a population of commercial pigs scored for backfat skatole levels, there was evidence of association between a SNP in the CYP2E1 promoter and skatole deposition, although there was no significant association between this SNP and skatole levels in the experimental cross.