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Polymorphism, linkage mapping and expression pattern of the porcine skeletal muscle glycogen synthase ( GYS1 ) gene
Author(s) -
Zuo B.,
Xiong Y. Z.,
Deng C. Y.,
Su Y. H.,
Wang J.,
Lei M. G.,
Li F. E.,
Jiang S. W.,
Zheng R.
Publication year - 2005
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.2005.01286.x
Subject(s) - biology , skeletal muscle , restriction fragment length polymorphism , single nucleotide polymorphism , glycogen synthase , microbiology and biotechnology , gene , genetics , glycogen , polymerase chain reaction , endocrinology , genotype
Summary The glycogen synthase gene ( GYS1 ), which encodes the rate‐limiting enzyme for glycogen synthesis of skeletal muscle, is a promising candidate gene for traits related to skeletal muscle in pigs. In this study, a G/A single nucleotide polymorphism in GYS1 intron 7 detected as a Fok I PCR‐restriction fragment length polymorphism (PCR‐RFLP) showed allele frequency differences among five Chinese indigenous pig breeds and three western commercial pig breeds. Linkage analysis assigned the gene GYS1 to marker interval SW1302 – SW1473 on SSC6 in a three‐generation Meishan X Large White reference family. The results of association analysis and interval mapping suggested that the Fok I PCR‐RFLP polymorphism might be linked with the quantitative trait loci affecting carcass traits detected on SSC6 in the F 2 intercross pedigree. The reverse transcriptase‐polymerase chain reaction revealed that the porcine GYS1 gene was expressed in spleen, lung, liver, kidney, small intestine, skeletal muscle, heart and stomach, with the highest expression in skeletal muscle.