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Genetic testing for feline polycystic kidney disease
Author(s) -
Grahn R. A.,
Biller D. S.,
Young A. E.,
Roe B. A.,
Qin B.,
Lyons L. A.
Publication year - 2004
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.2004.01213.x
Subject(s) - population , reproduction , medicine , library science , family medicine , veterinary medicine , biology , ecology , environmental health , computer science
Microsatellite primers developed from the sequence are PKD1UCD1F: 5¢-TTAAGCATTTACCCGGCATC-3¢ and PKD1UCD1R: 5¢-CATCCAGTCCAGTCCTTGT-3¢; PKD1UCD2F: 5¢-GCAGGGACTGTGAGGTTG-3¢ and PKD1UCD2R: 5¢-GCAGGGACTGTGAGGTTG-3¢. Sub-clones of the PKD2 clone were screened with a (CA)17 probe. Primer sequences for the PKD2derived microsatellite (GenBank accession number AY727857) are PKD2UCD1F: 5¢-GGAAATGCCCCAGTG-3¢ and PKD2UCD1 R: 5¢-TGGGTCTCTAGTCTGCTGTATGA-3¢. PKD1UCD2 and PKD2UCD1 have (CA)16 and (CA)19 repeats, respectively, whereas PKD1UCD1 is a (TTG)10 repeat. Two of the original seven pedigrees (190 cats, Families 2 and