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FISH mapping and sequence analysis of 87 porcine BAC clones
Author(s) -
Anistoroaei R.,
Cirera S.,
Jørgensen C. B.,
Fredholm M.,
Christensen K.
Publication year - 2004
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.2003.01083.x
Subject(s) - biology , fish <actinopterygii> , genetics , computational biology , sequence (biology) , sequence analysis , gene , fishery
Summary Ninety‐one bacterial artificial chromosomes (BAC) clones, selected effectively at random from our library, were used as probes for fluorescence in situ hybridization. Of these, 87 clones gave a specific signal in one, two or three different pair(s) of swine metaphase chromosomes. The ends of 35 BAC clones were sequenced in order to obtain information for comparative mapping. Fifteen of them gave useful comparative mapping information.