Premium
A radiation hybrid map of chicken chromosome 15
Author(s) -
Jennen D. G. J.,
Crooijmans R. P. M. A.,
Morisson M.,
Grootemaat A. E.,
Der Poel J. J.,
Vignal A.,
Groenen M. A. M.
Publication year - 2004
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.2003.01082.x
Subject(s) - radiation hybrid mapping , biology , gene mapping , chromosome , genetics , microsatellite , gene , gene map , sequence tagged site , genome , contig , allele
Summary We have constructed a radiation hybrid (RH) map of chicken chromosome (GGA) 15. This map can be used as a resource to efficiently map genes to this chromosome. The map has been developed using a 6000 rad chicken–hamster whole‐genome radiation hybrid panel (ChickRH6). In total, six microsatellite loci, 18 sequence tagged sites (STSs) from BAC end sequences and 11 genes were typed on the panel. The initial framework map comprised eight markers, and an additional 23 markers were then added to generate the final map. The total map length was 334 centiRay 6000 (cR 6000 ). The estimated retention frequency for the data set was 18%. Using an estimated physical length of 21 Mb, the ratio between cR 6000 and physical distance over GGA15 was estimated to be 0.063 Mb/cR 6000 . The present map increases the marker density and the marker resolution on GGA15 and enables fast mapping of new chicken genes homologous to genes from human chromosomes 12 and 22.