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Application of New World Camelidae microsatellite primers for amplification of polymorphic loci in Old World camelids
Author(s) -
Jianlin H,
Mburu D,
Ochieng J,
Kaufmann B,
Rege J E O,
Hanotte O
Publication year - 2000
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.2000.00683.pp.x
Subject(s) - livestock , animal production , geography , biology , library science , agricultural science , forestry , zoology , computer science
PCR condition: A polymarese chain reaction (PCR) amplification was performed using 20 ng of somatic cell hybrid panel DNAs, 1 X PCR buffer (Perkin Elmer), 2 mM MgCl2, 40 mM each of dNTPs, 0.5 unit of AmpliTaq polymerase (Perkin Elmer), and 400 nM of each primer in a 25 ml of reaction volume. The PCR cycling conditions included an initial denaturation of 10 min at 95 °C, followed by 40 cycles of 30 s at 95 °C, 30 s at 60 °C, and 1 min at 72 °C, with a final 20 min extension at 72 °C using a PTC-100 Programmable Thermal Controller (MJ Research, Inc).