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AFLP™ markers for DNA fingerprinting in cattle
Author(s) -
AjmoneMarsan P,
VecchiottiAntaldi G,
Bertoni G,
Valentini A,
Cassandro M,
Kuiper M
Publication year - 1997
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.1997.00204.x
Subject(s) - amplified fragment length polymorphism , biology , genetics , primer (cosmetics) , dna profiling , polymerase chain reaction , restriction enzyme , genomic dna , genotype , restriction fragment length polymorphism , genetic marker , cleaved amplified polymorphic sequence , taq polymerase , dna , microbiology and biotechnology , genetic diversity , gene , population , chemistry , demography , organic chemistry , sociology , thermus aquaticus
This work reports on use of the recently described amplified fragment length polymorphism (AFLP) technology for DNA fingerprinting in cattle. The AFLP technology produces molecular markers through the high‐stringency polymerase chain reaction (PCR)‐amplification of restriction fragments that are ligated to synthetic adapters and amplified using primers, complementary to the adapters, which carry selective nucleotides at their 3' ends. While, for plants, the double digestion of genomic DNA with Eco RI and Mse I is suggested, in mammals the enzyme combination Eco RI/ Taq I produces clearer and more polymorphic AFLP patterns. In a sample of 47 Italian Holstein genotypes, 16 Eco RI/ Taq I primer combinations identified 248 polymorphic bands in a species known for its low level of restriction polymorphism. In spite of the low information content carried by each AFLP polymorphism (average polymorphism information content = 0·31), the number of fragments revealed by each primer combination increased significantly the level of genetic information gained in each experiment. AFLP patterns are reproducible in independent experiments and polymorphic fragments segregate in cattle families according to Mendelian rules.