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Sequence analysis of MHC class I α2 domain exon variants in one diploid and two haploid Atlantic salmon pedigrees
Author(s) -
Grimholt U,
Olsaker I,
Lingaas F,
Lie Ø
Publication year - 1997
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.1997.00183.x
Subject(s) - biology , genetics , haplotype , ploidy , salmo , pedigree chart , major histocompatibility complex , locus (genetics) , allele , exon , gene , fishery , fish <actinopterygii>
Genetic diversity in the second domain exon of Atlantic salmon ( Salmo salar ) major histocompatibility complex (Mhc) class I was investigated in two dams and nine of their haploid offspring by means of polymerase chain reaction (PCR) and DNA sequence analysis. A similar study was also performed on nine diploid offspring from one of these dams. The complex segregation patterns and sequence similarities between variants make definitive allele, haplotype and locus assignments difficult. There are, however, indications of six Mhc‐ Sasa class I loci and a fairly well‐defined haplotype of four variants. One non‐polymorphic variant present in most specimens could be a salmon analogue to the human non‐classical loci.