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Extensive genomic conservation of cattle microsatellite heterozygosity in sheep
Author(s) -
Gortari M J de,
Freking B A,
Kappes S M,
Leymaster K A,
Stone R T,
Beattie C W,
Crawford A M
Publication year - 1997
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.1997.00153.x
Subject(s) - biology , loss of heterozygosity , microsatellite , locus (genetics) , genetics , allele , primer (cosmetics) , genetic marker , genetic linkage , gene , chemistry , organic chemistry
We report the evaluation of 1036 bovine microsatellite primer pairs for their suitability as linkage markers in sheep. Approximately 58% (605/1036) of bovine primer pairs amplified a locus in sheep. Sixty‐seven per cent (409/605) of amplified loci were detected as polymorphic. Marker heterozygosity, allele number and range of allele sizes were significantly lower in sheep than cattle sampled in this study. However, median fragment size was similar. These data suggest that high‐resolution comparative linkage maps between closely related species can be constructed relatively efficiently.