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Chicken red blood cells as a substrate for direct polymerase chain reaction
Author(s) -
Khatib H.,
Gruenbaum Y.
Publication year - 1996
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.1996.tb01177.x
Subject(s) - polymerase chain reaction , biology , microbiology and biotechnology , polymerase chain reaction optimization , real time polymerase chain reaction , dna , polymerase , genetics , gene , nested polymerase chain reaction
Summary A protocol is presented for direct polymerase chain reaction (PCR) amplification of DNA from chicken nucleated red blood cells. Chicken blood in EDTA was found to have a strong inhibitory effect on the PCR. Consequently, PCR using this protocol should be performed only on a narrow range of blood volumes, from 0125 to 025 |ll.