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Isolation and characterization of 45 polymorphic microsatellites from the bovine genome
Author(s) -
Ma R. Z.,
Russ I.,
Park C.,
Heyen D. W.,
Beever J. E.,
Green C. A.,
Lewin H. A.
Publication year - 1996
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.1996.tb01175.x
Subject(s) - microsatellite , biology , genetics , bovine genome , genomic library , tandem repeat , genotyping , genome , genomic dna , locus (genetics) , mendelian inheritance , microbiology and biotechnology , allele , dna , gene , genotype , base sequence
Summary A small‐insert bovine genomic library was constructed in pBluescript II SK(+) and enriched for microsatellites by selective rescue of single‐stranded pBluescript DNA carrying (CA) n /(TG) n tandem repeats. Approximately 50% of the clones in the enriched library contained (CA) n repeats or CA‐rich sequences. Sequencing of clones selected for (CA) n repeats resulted in the identification and characterization of 45 (CA) n polymorphic microsatellites. Genotyping in 9 large paternal half‐sib families indicated that 40 of these microsatellite markers exhibit autosomal Mendelian inheritance. The numbers of alleles range from 2 to 18, with an average of 6‐3 per locus. The polymorphic microsatellite markers we have identified and characterized will contribute to the construction of a high‐resolution linkage map of bovine genome.