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Characterization and linkage mapping often sheep microsatellite markers derived from a sheep x hamster cell hybrid
Author(s) -
Lumsden J M,
Lord E A,
Montgomery G W
Publication year - 1996
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.1996.tb00953.x
Subject(s) - biology , cosmid , microsatellite , genetics , genomic library , genomic dna , chromosome , gene mapping , genetic linkage , microbiology and biotechnology , genetic marker , dna , gene , allele , base sequence
Summary A cosmid library has been constructed from a sheep x hamster cell hybrid containing sheep chromosome t 1 , rob (6;24). Clones containing sheep DNA were identified by hybridizing to a total sheep genomic DNA probe. Small fragments (<500 bp) containing (AC) n microsatellites were subcloned and sequenced. Ten microsatellite markers were characterized and six were mapped back to chromosomes 6 and 24. The remaining microsatellites mapped to chromosome 26, which was shown to be present in a small proportion of cells of the cell line.