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Restriction fragment length polymorphism in amplification products of the bovine butyrophilin gene: assignment of bovine butyronhilin to bovine chromosome 23
Author(s) -
Taylor C,
Everest M,
Smith C
Publication year - 1996
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.1996.tb00948.x
Subject(s) - biology , sire , bovine milk , restriction fragment length polymorphism , genetics , gene , bovine genome , quantitative trait locus , restriction enzyme , microbiology and biotechnology , polymerase chain reaction , genome , biochemistry , zoology
Summary A polymorphism was identified in the bovine butyrophilin (BTN) gene by digesting poly‐merase chain reaction products with the restriction enzyme Hae III. This polymorphism was segregating in a Holstein‐Friesian sire selected as part of an ongoing study directed towards the identification of quantitative trait loci affecting milk composition. Screening of a half‐sib family established for the heterozygous sire allowed the localization of BTN to bovine chromosome 23 (BTA23).