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A MspI polymorphism in the bovine ornithine decarboxylase gene and its possible association with selection for milk production in Holstein bulls
Author(s) -
Yao J.,
Zadworny D.,
Kuhnlein U.,
Aggrey S. E.,
Hayes J. F.
Publication year - 1996
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.1996.tb00491.x
Subject(s) - biology , allele , restriction fragment length polymorphism , allele frequency , genetics , polymorphism (computer science) , genotype , polymerase chain reaction , gene
Summary A polymerase chain reaction (PCR)‐based method for detection of a MspI‐restriction fragment length polymorphism (RFLP) in the bovine ornithine decarboxylase gene was developed, and the allele frequencies of the polymorphism in two groups of Holstein bulls representing progeny‐tested bulls during the 1950s‐1960s and 1980s, respectively, were estimated. The frequencies of the MspI(‐) allele were 0.229 and 0.077 and that of MspI(+) were 0.771 and 0–923 in the progeny‐tested bulls of the 1950s‐1960s and 1980s, respectively. The difference in allele frequencies between the two groups was statistically significant (P< 0.005). Genetic drift could be responsible for the changes in allele frequencies; however, it could also be possible that selection for milk production was associated with the changes of the allele frequencies in the two bull populations.