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A new DNA marker, U15557, is linked to protease inhibitor and adenylate kinase‐1 in the laboratory opossum, Monodelphis domestica
Author(s) -
Perelygin A. A.,
Samollow P. B.,
Perelygina L. M.,
Cherry L. M.,
Mahaney S. M.,
VandeBerg J. L.
Publication year - 1996
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.1996.tb00479.x
Subject(s) - biology , microbiology and biotechnology , monodelphis domestica , genomic dna , opossum , genetics , polymerase chain reaction , dna , restriction fragment length polymorphism , genetic marker , gene , anatomy
Summary A 323‐bp DNA fragment (U15557) was isolated, cloned, and sequenced after polymerase chain reaction (PCR) amplification from Monodelphis domestica genomic DNA. AHindIII restriction fragment length polymorphism was identified in this species using the U15557 PCR. fragment as a hybridization probe. DNA samples exhibited either a 6.4kb band, a 7.2 kb band, or both bands simultaneously. Behaviour of these two variants in family studies was consistent with codominant autosomal inheritance. Linkage between this marker and the loci encoding protease inhibitor (PI) and adenylate kinase 1 (AK1) was found in M. domestica.