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Porcine alpha‐1‐antitrypsin (PI): cDNA sequence, polymorphism and assignment to chromosome 7q2.4‐ > q2.6
Author(s) -
Archibald A. L.,
Couperwhite S.,
Mellink C. H. M.,
LahbibMansais Y.,
Gellin J.
Publication year - 1996
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.1996.tb00472.x
Subject(s) - biology , complementary dna , microbiology and biotechnology , locus (genetics) , genetics , gene mapping , bamhi , restriction fragment length polymorphism , coding region , restriction enzyme , chromosome , gene , genotype
Summary A cDNA clone encoding the complete coding sequence for porcine alpha‐1‐antitrypsin (or α 1 ‐protease inhibitor, PI ) was isolated and its DNA sequence determined. The cDNA is assumed to encode alpha‐1‐antitrypsin on the basis of its sequence similarity to the corresponding cDNAs for human, baboon, rat, mouse, sheep and cow. The porcine cDNA clone was used in conjunction with Bam HI, KpnI, MspI, SacI, TuqI and XbaI to develop restriction fragment length polymorphism‐based genetic markers for linkage mapping in pigs. The cDNA has also been used to map the porcine PI locus to chromosome 7q2.4‐ > q2.6 by radioactive in situ hybridization. Thus, the PI locus has been added to the developing physical and genetic maps of the porcine genome.