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Sheep linkage mapping: RFLP markers for comparative mapping studies
Author(s) -
Montgomery G W,
Penty J M,
Henry H M,
Sise J A,
Lord E A,
Dodds K G,
Hill D F
Publication year - 1995
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.1995.tb03252.x
Subject(s) - biology , restriction fragment length polymorphism , linkage (software) , gene mapping , genetics , genetic linkage , computational biology , genetic marker , polymerase chain reaction , gene , chromosome
Summary Restriction fragment length polymorphisms (RFLPs) detected using cDNA probes for conserved genes provide an important set of markers that anchor or link syntenic groups in a range of divergent mammalian species. DNA probes from sheep, cattle, pig, human and mouse were screened against sheep DNA samples and 24 new RFLP markers for sheep were identified. Among the loci tested, 22 had a homologue that has been mapped in humans. An RFLP for fibronectin (FN1) was linked to α‐inhibin (INHA) at a distance of 5cM. The FN1 locus has been assigned to sheep chromosome 2q41–q44 and linkage between FN1 and INHA assigns INHA to the same chromosome in sheep. In addition to the new loci reported here, 28 RFLPs have been published previously by this group and these are collated together with RFLPs published from other laboratories. RFLPs have been reported for 86 loci in sheep. Fifty‐four loci have been mapped to 16 different chromosomes.