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A rapid PCR‐based test for the endogenous viral element ev 3 of chickens
Author(s) -
Benkel B. F.,
Perreault J,
Gag C,
Conklin K
Publication year - 1995
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.1995.tb03161.x
Subject(s) - biology , endogeny , virology , genetics , test (biology) , computational biology , paleontology , endocrinology
Summary A short fragment of chicken genomic DNA encompassing the insertion site of the endogenous avian leucosis viral element ev 3 was isolated using the inverse polymerase chain reaction (inverse PCR) technique. The nucleotide sequence of the unoccupied site was used to design PCR primers that can be used to unambiguously determine the genetic status of any chicken, with respect to ev 3. Screening of a small number of individuals from exotic breeds of chickens suggested that the frequency of ev 3 is highly variable. The ev 3 integration site shows a high degree of sequence homology with the macrophage‐specific tyrosine kinase gene, bmk , in mice.