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Organization of the sex‐linked late‐feathering haplotype in chickens
Author(s) -
Iraqi F,
Smith E J
Publication year - 1995
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.1995.tb03153.x
Subject(s) - biology , haplotype , white (mutation) , genetics , microbiology and biotechnology , southern blot , dna , repeated sequence , genotype , homologous chromosome , chromosome , polymerase chain reaction , gene , genome
Summary Nucleotide sequence analysis of polymerase chain reaction products confirmed that ev 21 integrated into one of two large homologous elements on the Z chromosome of late‐feathering (LF) White Leghorn chickens. Southern blots of Not I‐, Nae I‐, Ksp I‐ and Bam HI‐digested DNA from early‐feathering (EF) and LF White Leghorns, that had been hybridized with a probe that flanks ev 21, indicated a 180 kb duplication of an unoccupied repeat in the LF genotype of White Leghorns. A Ksp I fragment that carries ev 21 was about 32 kb smaller than the Ksp I fragment found in EF DNA. In the evolution of LF, retroviral insertion into one of two large repeats and a 32 kb deletion may have generated LF.