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Polymorphic CAC/T repetitive sequences in the pig genome 1
Author(s) -
Coppieters W,
Weghe A,
Depicker A,
Coppieters J,
Peelman L,
Zeveren A,
Bouquet Y
Publication year - 1995
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.1995.tb02667.x
Subject(s) - biology , genetics , polymerase chain reaction , southern blot , microbiology and biotechnology , genome , genomic dna , dna , repeated sequence , gene
Summary Three genomic clones were isolated from a sizeselected pig DNA library by hybridization with a DNA‐fingerprint probe. Analysis at the sequence level revealed that all three clones contain interrupted stretches of triplet repeats mainly composed of CAC and CAT triplets. Evaluation of the corresponding loci for polymorphism by Southern blot hybridization showed considerable length variation. For two loci the polymorphism was also demonstrated by polymerase chain reaction (PCR) amplification. The PiGMaP reference pedigree was typed for all three loci.