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SSCP analysis of pig mitochondrial DNA D‐loop region polymorphism
Author(s) -
Takeda K,
Onishi A,
Ishida N,
Kawakami K,
Komatsu M,
Inumaru S
Publication year - 1995
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.1995.tb02666.x
Subject(s) - biology , d loop , single strand conformation polymorphism , mitochondrial dna , microbiology and biotechnology , polymerase chain reaction , genetics , polymorphism (computer science) , dna , gene , genotype
Summary The sequence polymorphism that occurs in the mitochondrial DNA (mtDNA) displacement (D)‐loop region is useful as a cytoplasmic DNA marker. We cloned the mtDNA D‐loop regions of five breeds of pig by polymerase chain reaction (PCR) and determined their sequences. The sequence diversities in D‐loop regions among five breeds of pig were located in the starting area of heavy‐strand replication. From these sequences, we designed primers for PCR‐mediated single‐strand conformation polymorphism (PCR‐SSCP) analysis that amplified the most polymorphic 227 bp fragment of the D‐loop region. The results of PCR‐SSCP analysis clearly showed that four types of polymorphism (A to D) are found in Landrace (A), Large White (A, B), Duroc (A), Göttingen miniature pig (B) and Meishan (C, D). The same polymorphisms were also detected from each porcine embryo by this method. Our results show that PCR‐SSCP analysis is useful in detecting polymorphisms in the D‐loop region of pigs and pig embryos.