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Discrimination between major histocompatibility complex class II DQ and DR locus products in cattle
Author(s) -
Dutia B M,
MacCarthyMorrogh L,
Glass E J,
Knowles G,
Spooner R L,
Hopkins J
Publication year - 1995
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.1995.tb02643.x
Subject(s) - biology , major histocompatibility complex , monoclonal antibody , mhc class ii , microbiology and biotechnology , locus (genetics) , genetics , mhc class i , antigen , antibody , immunology , gene
Summary We have used a panel of anti‐major histocompatibility complex (MHC) class II monoclonal antibodies (mAbs) and have assessed their specificity for the products of the individual bovine MHC (BoLA) class II subregions. The mAbs identified two distinct class II molecules by affinity purification and ELISA. Two‐dimensional immunoblotting confirmed these data and NH 2 ‐terminal sequencing of the purified class II α chains of one member of each group identified the subregion specificity of the mAbs. The mAbs VPM36, TH22A and TH81A are specific for BoLA DQ, whereas VPM54, TH14B and J11 are specific for BoLA DR. SW73.2 reacts with both MHC subgroups of all cattle tested.