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Features of the DNA fingerprinting probe pITZ1
Author(s) -
Huebscher K J,
Dolf G,
Frey J,
Stranzinger G,
Gaillard C
Publication year - 1995
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.1995.tb02615.x
Subject(s) - biology , plasmid , minisatellite , genetics , dna profiling , hybridization probe , molecular probe , puc19 , genomic library , tandem repeat , polymerase chain reaction , dna , microbiology and biotechnology , genomic dna , repeated sequence , microsatellite , genome , gene , base sequence , allele
Summary Stringently controlled plasmids generate DNA fingerprint patterns in mammals when used at low hybridization temperatures. In order to develop a probe for use in paternity testing in cattle we screened a bovine, partial genomic plasmid library with the PCR‐amplified ori region of plasmid P1. Of eight isolated clones one generated strong band patterns at high stringency in various mammalian species (data not shown). Sequence analysis revealed an imperfect, compound dinucleotide repeat region, which was PCR‐amplified and cloned into the plasmid vector pUC19. Fingerprint results generated by this probe (termed pITZ1) in cattle are compared with the results generated by VNTR‐probe pV47, which itself was developed by screening a human chromosome 16 library with tandem repeats of bacteriophage M13. Probe pITZ1 is useful in conjunction with other VNTR‐probes for DNA fingerprinting in cattle and donkey populations. The dinucleotide repeat region responsible for the band patterns generated with pITZ1 is close to an Alu ‐like sequence, which may be involved in eukaryotic replication mechanisms.