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Minisatellite DNA markers in the chicken genome
Author(s) -
Bruford M. W.,
Hanotte O.,
Burke T.
Publication year - 1994
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.1994.tb00529.x
Subject(s) - minisatellite , biology , genetics , genome , dna , computational biology , genetic marker , dna profiling , repeated sequence , microsatellite , gene , allele
Summary The isolation of chicken minisatellites is reported. Two charomid libraries were constructed from fractions of size‐selected MboI‐digested genomic DNA. A total of 5985 colonies were screened with the multilocus probes 33.6 and 33.15; 137 positives were obtained (2.35%). A total of 55 clones were tested against four unrelated chickens; 30 revealed variable single locus patterns. More detailed characterization was made of 15 probes, which were tested against four families comprising a total of 53 offspring. Of parent/offspring comparisons 51% were informative for segregation. No mutations were detected. Three minisatellites were linked on the same autosome, implying that these loci may occur in clusters, and one was Z chromosome‐linked. Heterozygosity and allelic variability were measured in 67 individuals to several different strains and breeds. Mean heterozygosity ranged from 50 to 84%. Minisatellite loci appear to be highly variable in the chicken and should provide highly informative markers in intraspecific crosses in genome mapping studies.