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Restriction fragment length polymorphism (RFLP) of exon 2 of the MhcBibi‐DRB3 gene in American bison ( Bison bison )
Author(s) -
Morris B G,
Spencer M C,
Stabile S,
Dodd J N
Publication year - 1994
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.1994.tb00409.x
Subject(s) - restriction fragment length polymorphism , biology , restriction enzyme , terminal restriction fragment length polymorphism , restriction site , genetics , microbiology and biotechnology , exon , restriction fragment , polymerase chain reaction , polymorphism (computer science) , allele , gene
Summary Polymerase chain reaction (PCR) primers specific to exon 2 of the bovine lymphocyte antigen ( BoLA)‐DRB3 gene were successfully used to amplify the equivalent region in 469 American bison ( Bison bison ). In domestic cattle, alleles of DRB3 are assigned through a restriction fragment length polymorphism (RFLP) analysis of the patterns of fragment lengths observed after digestion with the restriction enzymes Rsa I, Bst YI and Hae III. In bison, using the same procedure, the observed RFLP patterns provided evidence for the strong conservation of restriction sites previously reported in cattle.