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A rapid protocol for sexing chick embryos (Gallus g. domesticus)
Author(s) -
Clinton M.
Publication year - 1994
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.1994.tb00374.x
Subject(s) - sexing , biology , w chromosome , polymerase chain reaction , embryo , proteinase k , microbiology and biotechnology , dna , genetics , andrology , chromosome , gene , karyotype , medicine
Summary A method for establishing the sex of chick embryos before the appearance or morphological differences between males and females has been developed. DNA was isolated from 5–7‐day‐old embryos by proteinase K digestion and subjected to polymerase chain reaction (PCR) amplification with W‐chromosome specific primers. Sexing can be achieved within 1 day using as little as 1 ng template DNA.