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Divergent selection for antibody production in common carp (Cyprinus carpio L.) using gynogenesis
Author(s) -
Wiegertjes G F,
Stet R J M,
Muiswinkel W B
Publication year - 1994
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.1994.tb00201.x
Subject(s) - cyprinus , biology , common carp , selection (genetic algorithm) , carp , fish <actinopterygii> , fishery , genetics , zoology , artificial intelligence , computer science
Summary A base population (n = 101) of carp, consisting of a single hybrid cross, was immunized with the hapten‐carrier complex DNP‐KLH. to perform a divergent selection for antibody response. Measurement of the DNP‐specific antibody response at 12 and 21 days postimmunization, allowed the classification of a low number of individual carp as early/high (10%) or late/low (13%) responders. Three individuals defined as early/high and three defined as late/low responding, were gynogenetically reproduced to obtain corresponding homozygous progenies within one generation only. Upon immunization with DNP‐KLH, the antibody response was found to be significantly higher in the early/high responder homozygous offspring. Although the homozygosity of the offspring apparently caused a (s)lower antibody response (compared with the base population), the differences between the high and low responder offspring do indicate a genetic influence on the antibody response. The realized heritability (h2) for antibody production was estimated at 0.37 ± 0.36. The present study provides the basis for a divergent selection of homozygous inbred carp lines with a genetically controlled difference in antibody response. These inbred lines will allow us to investigate relationship(s) between immune responsiveness and resistance to infectious diseases in fish.