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Expression pattern, genomic cloning and RFLP analyses of the swine PIT‐1 gene
Author(s) -
Yu TP,
Schmitz C B,
Rothschild M F,
Tuggle C K
Publication year - 1994
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.1994.tb00198.x
Subject(s) - restriction fragment length polymorphism , biology , genetics , genomic dna , ecori , restriction enzyme , polymerase chain reaction , complementary dna , microbiology and biotechnology , restriction site , gene , restriction map , plasmid
Summary The swine PIT‐1 POU domain cDNA was used to study PIT‐1 expression and to clone the PIT‐1 genomic region to identify additional PIT‐1 polymorphisms. PIT‐1 was expressed only in the pituitary, confirming the swine cDNA identity. To study the PIT‐1 genomic region, a clone containing 13.7 kb DNA was isolated. EcoRI fragments hybridizing to PIT‐1 were sequenced, and the 3′ portion of PIT‐1 gene identified. Primers for polymerase chain reaction (PCR)‐restriction fragment length polymorphism (RFLP) analysis were then designed. Restriction analysis of PCR products identified a RsaI RFLP. In contrast to other PIT‐1 RFLPs, the RsaI PCR‐RFLP was widely distributed in American breeds. Thus, the RsaI RFLP and the previously reported PIT‐1 RFLPs allow detection of PIT‐1 alleles in most, if not all, breeds currently used in the US and European reference/resource pig mapping families.