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A study of variability in the MHC class II β1 and class I α2 domain exons of Atlantic salmon, Salmo salar L.
Author(s) -
Grimholt U,
Olsaker I,
Lindstrøm C de Vries,
Lie Ø
Publication year - 1994
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.1994.tb00103.x
Subject(s) - salmo , biology , exon , genetics , mhc class i , mhc class ii , genomic dna , polymerase chain reaction , gene , major histocompatibility complex , fish <actinopterygii> , fishery
Summary Variability in the most extracellular exons of Atlantic salmon MHC‐Sasa class I and class II was studied by polymerase chain reaction (PCR) amplification followed by sequencing. The domains studied were class I α2 and class II β1. The material used was genomic DNA of fish, mainly derived from the major Norwegian breeding pool, supplemented with some material from a minor breeder and a local river strain. The analysis revealed extensive variation, most individuals being heterozygous with at least two variants.

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